Petite H(1), Frei V, Huc A, Herbage D. Author information: (1)Coletica, Lyon, France. Cross-linking of collagen-based biomaterials increases their strength and. By DANIEL HERBAGE, JOSETTE BOUILLET and JEAN-CLAUDE Solubilization of collagen from bovine articular cartilage with pepsin. The formation of new bonds between the denatured collagen chains and the proteoglycans can explain the D. Herbage, A. Huc, D. Chabrand, M.C. Chapuy.

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Cytological evaluation of capsular tissue surrounding TCPL implant in adult rats. After determining the optimal conditions of cross-linking with DPPA, we compared the efficiency of this protocol with that using hydrazine and with the classical glutaraldehyde treatment.

Use of diphenylphosphorylazide for cross-linking collagen-based biomaterials.

Topics Discussed in This Paper. DPPA-treated pericardium had a resistance cllagen collagenase digestion similar to that of glutaraldehyde- or hydrazine-treated pericardium. Glycosaminoglycans accumulated in the matrix, up to 1.

In order to validate and quantitate the extent of reaction, the degree of cross-linking was determined by the measure of the free primary amino group content of the samples.

AliDavid J Mooney Current topics in microbiology and immunology Cross-linking of collagen-based biomaterials increases their strength and persistence in vivo. RosenthalGabriele J Koehler Anticancer research Allogeneic Mesenchymal Stem Cells and Biomaterials: Mineral deposition Clolagen deposition Articular cartilage repair Collagen-based biomaterial Collagen synthesis.

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Methods for the treatment of collagenous tissues for bioprostheses.

Both supports were always present during the study and a partial decrease in size and weight was detected only with control sponges, both seeded and unseeded. Citations Publications citing this paper. Wednesday, December 13, – 2: Cell proliferation was only noted in the 10 6 cells-seeded sponges 4-fold increase after 4 weeks of culture. Ham A Benghuzzi Biomedical sciences instrumentation Similar decreases in free primary amino group content and increases in thermal stability were obtained for collagen films treated with DPPA, glutaraldehyde, or hydrazine.

Collagen-based biomaterials in the form of sponges bovine type I collagen, both native and cross-linked by treatment with diphenylphosphorylazide, noted control and DPPA sponges respectively were tested as three-dimensional scaffolds to support chondrocyte proliferation with maintenance of the phenotype in order to form neocartilage. Collagen-based biomaterials as 3D scaffold for cell cultures: Aluminum Oxide Search for additional papers on this topic.

Files produced by the author s. Treatment of native bovine pericardium with 0. Application of Collagen Scaffold in Tissue Engineering: References Publications referenced by this paper.

Collagen – References

Mouse fibroblasts in long-term culture within collagen three-dimensional scaffolds: These in vitro results indicate that both collagen matrices can support the development of tissue engineered cartilage. Recently, we described an efficient cross-linking process via the formation of acyl azide groups on methylated carboxyl groups of collagen using hydrazine and nitrous acid referred to here as the hydrazine method.

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ChevallayDr D.

Native and DPPA cross-linked collagen sponges seeded with fetal bovine epiphyseal chondrocytes used for cartilage tissue engineering. Anne-Marie Freyria 1 AuthorId: Monday, October 29, – 9: Gelatine-Based Biomaterials and Their Biocompatibility: Howard The Journal of biological chemistry Daniel Herbage 1 AuthorId: These results were corroborated by resistance to bacterial collagenase digestion: Showing of 71 extracted citations.

BiomaterialsElsevier,22, pp.

herbge Vacanti Journal of cellular biochemistry. With the rapid development in tissue engineering, these different biomaterials have been used as three-dimensional scaffolds and cell transplant devices.

Collagen References

In this report, we propose a simpler, faster way to prepare acyl azide groups and to cross-link collagen-based biomaterials, using diphenylphosphorylazide DPPA as a reagent. Specific cartilage collagens types II and XI were deposited in the matrix throughout the culture collagej traces of type I collagen were noticed only in the culture medium after weeks and 4 weeks in the case of 10 6 and 10 7 cells-seeded sponges, respectively.

Cross-linking of dermal sheep collagen using a water-soluble carbodiimide. In the collaagen 7 cells-seeded sponges, mineral deposition, observed with unseeded sponges, was significantly decreased 2- to 3-fold.