The Elutrap system is designed to isolate nucleic acids and proteins from agarose or polyacrylamide gel slices by electroelution. Samples are purified with . azim58 – Official elutrap documentation. Elutrap protocol. “C:\kurt\storage\CIM Research Folder\DR\\\ELUTRAP\ “. The Elutrap, seen from above, is placed on a horizontal agarose gel electrophoresis chamber. Outer limits of the trap are defined with disposable membranes at.
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View my complete profile. Place it between the screw and the closed U insert in the smaller chamber of the elutrap device. Take the BT1 membrane out using forceps and clean thoroughly with DI water to remove all traces of glycerol. However, take precautions to keep the BT2 membrane dry for as long as possible.
Collecting the sample too slowly can result in collection of a greater volume of the buffer as the buffer continues to diffuse into the collection chamber. To clean, discard the buffer down the drain. Leakage can be detected by the appearance of water on the paper filter.
Whatman Elutrap Electroelution System, Whatman 10404090 Replacement Components BT1 Membranes
The Elutrap system is designed to isolate nucleic acids and proteins from agarose or polyacrylamide gel slices by electroelution. When I first started doing some research about the equipment, I found that Schleicher-schuell the company which initially came up with the elutrap was acquired by Whatman, and the protocol was no longer published online. Place the BT2 paper filter on the other surface of the closed U insert while taking care to keep it dry.
Take care not to crush the gel as this may impair elution efficiency. Gel pieces containing the purified template.
Under Ground Grease Traps : Aluline Ltd
Most of the nucleic acid is typically obtained from the first collection. Labnet E 35 well comb for use with 15cm gel system, 1. BT2 membrane — one per each device used. Newer Post Older Post Home.
If you can’t find what elutarp need, contact us to solve your problems. Cut out an adequate piece of dialysis membrane and place in the beaker containing the DI water.
Remember that the dialysis membrane is bi-layered. You can also explore other items in the Electrophoresis system power suppliesOther Lab ApparatusLab Apparatus categories yourself to try and find the perfect replacement for you! Remember that the red colored positively charged electrode is on the same end elutdap the collection chamber. Read more The Elutrap system can be used with other horizontal gel electrophoresis mamual.
When the channels are not occupied with a device, make sure the apertures do not align, elurrap off the flow of current through them.
A small beaker containing DI water — to place dialysis membrane prior to use. BT1 Membranes Whatman No.: Our site is powered by industry leading security standards for your protection. We apologize for any inconvenience. After collection of each sample, check the OD optical density of the buffer collected.
Run the unit at V for three hours. Add the gel pieces to the central elution chamber containing the 0. Finally, mqnual thoroughly with DI water. Fill the electrophoresis chamber to half full with 0. Please add ” labplanet.
The membrane placement is adjustable, allowing final elution trap volumes to be optimized for the particular assay. This is another protocol that I wrote for Dr Kim’s Lab, which proved to be very useful. If for any reason you decide that your purchase just isn’t for you, simply return the item within 30 days of receipt and we’ll cover the cost of return shipping.
Conclusion Organic solvents may damage the device and should be removed promptly.
Gel slices are placed in the middle of the Elutrap device and then placed elturap a horizontal electrophoresis chamber. Make sure that the inserts are tightly placed and rest on the designated slot.
Elutrap Electrophoresis Chamber
Always keep the BT1 and the dialysis membrane moist. Turn off the unit after completion of the elution. The advantages of this system over previously used techniques such as diffusion and simple electro-elution are a elutdap recovery rate, b high reproducibility, c high purity of recovered material and d the ease of use 1.